Used primarily in molecular biology and biochemistry research, this compound serves as a modified nucleoside. It is incorporated into DNA during synthesis to study DNA-protein interactions, as it reduces non-specific binding and enhances the stability of DNA structures. It is also employed in fluorescence-based assays, sequencing technologies, and PCR reactions for GC-rich templates to improve the accuracy of detecting nucleotide sequences and amplification efficiency. Additionally, it is utilized in the development of therapeutic oligonucleotides, where its modification helps in reducing immune responses and increasing the efficacy of the treatment.