6-Azauridine is primarily utilized in the field of medical research and treatment, particularly in oncology. It functions as an antimetabolite, interfering with the synthesis of nucleic acids, which is crucial for the proliferation of cancer cells. This compound is often employed in studies focused on understanding and inhibiting the growth of various types of tumors. Additionally, it has been explored for its potential antiviral properties, particularly in the treatment of certain viral infections where it can disrupt viral replication. In experimental settings, 6-Azauridine is also used to investigate cellular processes related to RNA metabolism and to study the effects of nucleotide analogs on cellular functions. Its role in these areas makes it a valuable tool in both therapeutic development and basic biological research.