2'-C-Methyluridine is primarily used in the development of antiviral medications, particularly for treating hepatitis C virus (HCV) infections. It serves as a key intermediate in the synthesis of nucleoside analogs, which are designed to inhibit viral replication by interfering with RNA synthesis. This compound is also explored in research for its potential application in creating therapies for other RNA viruses. Additionally, it plays a role in studies focused on understanding the mechanisms of nucleotide metabolism and antiviral resistance.