Used primarily in research settings, 2',3'-Dideoxyuridine serves as a nucleoside analog to study viral replication mechanisms, particularly in retroviruses. It helps in understanding the inhibition of viral DNA synthesis by acting as a chain terminator. This compound is also utilized in the development of antiviral drugs, providing insights into the design of more effective treatments for viral infections. Additionally, it plays a role in biochemical studies to explore nucleotide metabolism and enzyme interactions.