This compound is primarily utilized in the field of biochemistry and molecular biology as a modified nucleoside. It serves as a crucial component in the synthesis of oligonucleotides, particularly in the development of probes and primers for nucleic acid detection and amplification techniques like PCR. Its fluorinated structure enhances the stability and binding affinity of the synthesized oligonucleotides, making it valuable for diagnostic applications and research studies targeting specific genetic sequences. Additionally, it is employed in the study of enzyme mechanisms and nucleic acid interactions, providing insights into the biochemical pathways and functions of nucleic acids.