N6,N6-Dimethyladenosine is primarily used in biochemical research as a modified nucleoside. It plays a role in studying RNA modifications and their impact on gene expression and cellular function. This compound is valuable in understanding the mechanisms of RNA methylation, which is crucial for regulating mRNA stability, translation, and splicing. Additionally, it is utilized in the development of therapeutic strategies targeting RNA-related diseases, such as cancer and viral infections. Its application extends to the synthesis of modified nucleotides for probing RNA structure and function in molecular biology experiments.