N2,N2-Dimethylguanosine is primarily utilized in biochemical research, particularly in the study of RNA modifications and their functional roles. It serves as a key molecule for investigating the impact of methylation on RNA stability, structure, and interaction with proteins. This compound is also employed in the development of analytical methods to detect and quantify modified nucleosides in RNA samples, aiding in the understanding of epigenetic regulation and cellular processes. Additionally, it finds application in the synthesis of modified oligonucleotides for therapeutic and diagnostic purposes, particularly in the field of RNA-based therapeutics.