N4-Acetyl-2'-O-methylcytidine is primarily used in the field of RNA research and therapeutics. It serves as a modified nucleoside that enhances the stability and efficiency of RNA molecules. This modification is particularly valuable in the development of mRNA vaccines and therapeutic RNA, as it helps to reduce immunogenicity and improve translation efficiency. Additionally, it is employed in the study of RNA structure and function, aiding in the understanding of RNA interactions and modifications. Its application extends to the synthesis of oligonucleotides for diagnostic tools and research reagents, contributing to advancements in genetic and molecular biology studies.