Used in RNA research to study and modify RNA structures, enhancing stability and resistance to degradation. Applied in the development of therapeutic RNA molecules, such as mRNA vaccines and RNA-based drugs, to improve their efficacy and longevity in the body. Employed in the synthesis of modified nucleosides for probing RNA function and interactions in biochemical assays. Utilized in the design of antisense oligonucleotides and siRNA to enhance target specificity and reduce off-target effects. Supports advancements in gene therapy and personalized medicine by enabling precise RNA modifications for therapeutic applications.