N2-DMF-rG is primarily used in biochemical research and pharmaceutical development. It serves as a modified nucleoside, often employed in the synthesis of oligonucleotides for studying RNA structure and function. Its application extends to the development of therapeutic RNA molecules, such as antisense oligonucleotides and siRNA, where it enhances stability and binding affinity. Additionally, it is utilized in the exploration of gene expression mechanisms and the design of RNA-based drugs targeting specific genetic disorders or diseases. Its role in improving the efficacy and specificity of RNA therapeutics makes it a valuable tool in modern molecular biology and medicine.