Used primarily in the synthesis of oligonucleotides, particularly in the development of antisense drugs and therapeutic agents. It serves as a protected nucleoside building block in solid-phase synthesis, ensuring precise incorporation of modified cytidine into DNA or RNA sequences. The 2'-fluoro modification enhances stability and binding affinity, making it valuable in research targeting gene expression modulation and antiviral therapies. The DMT (dimethoxytrityl) group facilitates purification during synthesis, while the acetyl group protects the amine functionality, ensuring controlled and efficient reactions. Its application is crucial in creating tailored nucleic acid sequences for diagnostic tools, therapeutic interventions, and molecular biology studies.