1-Methylinosine is primarily used in biochemical research as a modified nucleoside. It plays a significant role in studying RNA modifications and their impact on gene expression and cellular functions. Researchers utilize it to explore the mechanisms of RNA methylation, which is crucial for understanding processes like mRNA stability, translation efficiency, and RNA-protein interactions. Additionally, it serves as a valuable tool in the development of therapeutic strategies targeting RNA-related diseases, such as cancers and viral infections. Its application extends to the synthesis of modified oligonucleotides for probing enzymatic activities and structural studies of RNA molecules.