8-Iodoguanosine is primarily used in biochemical research as a modified nucleoside. It serves as a key component in the study of nucleic acid structure and function, particularly in understanding the mechanisms of RNA and DNA interactions. This compound is often employed in the synthesis of labeled nucleotides for use in various molecular biology techniques, such as sequencing and hybridization assays. Additionally, it is utilized in the development of antiviral and anticancer agents, where its incorporation into nucleic acids can help in targeting specific cellular processes. Its iodine moiety also makes it useful in radiolabeling studies, enabling the tracking of nucleotide incorporation and metabolism in biological systems.