Used in the synthesis of oligonucleotides, particularly in solid-phase synthesis where it serves as a protecting group for the 5'-hydroxyl group of nucleosides. This protection is crucial during the stepwise assembly of DNA or RNA sequences, ensuring the correct addition of nucleotides without unwanted side reactions. After the synthesis, the trityl group can be selectively removed under mild acidic conditions, allowing for the continuation of the sequence assembly or the final deprotection of the oligonucleotide. Its application is essential in the production of primers, probes, and other nucleic acid-based tools used in molecular biology, diagnostics, and therapeutic research.