N4-Methyl-2'-deoxycytidine is primarily used in the field of molecular biology and biochemistry for studying DNA modifications and their functional roles. It serves as a modified nucleoside that helps researchers understand the impact of methylation on DNA structure, stability, and interactions with proteins. This compound is particularly valuable in epigenetic research, where it aids in exploring how methylation patterns influence gene expression and cellular processes. Additionally, it is utilized in the synthesis of modified oligonucleotides for probing DNA repair mechanisms and enzymatic activities. Its applications also extend to the development of diagnostic tools and therapeutic strategies targeting epigenetic regulation in diseases such as cancer.