Adenosine-5'-carboxylic acid is primarily used in biochemical research and pharmaceutical development. It serves as a key intermediate in the synthesis of nucleoside analogs, which are essential in creating antiviral and anticancer drugs. Its structure allows for modifications that can enhance drug efficacy and specificity. Additionally, it is employed in studies exploring enzyme mechanisms and nucleotide metabolism, providing insights into cellular processes and potential therapeutic targets. Its role in the development of novel therapeutics makes it valuable in advancing medical treatments.