Used in the synthesis of oligonucleotides for research in molecular biology and biochemistry. It serves as a modified nucleoside to study DNA interactions and enzymatic processes. Often employed in the development of antisense oligonucleotides and therapeutic agents targeting genetic disorders. Its fluorine substitution enhances stability and binding affinity, making it valuable in probing nucleic acid structures and functions. Also utilized in diagnostic assays and as a tool in drug discovery to investigate gene expression and regulation.