N2-Methylguanosine is primarily used in the field of biochemistry and molecular biology as a marker for studying RNA modifications. It plays a significant role in research related to RNA metabolism and function, particularly in understanding the mechanisms of post-transcriptional modifications. This chemical is also utilized in the development of diagnostic tools for detecting RNA-related diseases and in the study of epigenetic regulation. Additionally, it aids in exploring the impact of RNA modifications on gene expression and cellular processes, contributing to advancements in genetic and therapeutic research.