This chemical is primarily used in the synthesis of oligonucleotides, particularly in the field of molecular biology and genetic research. It serves as a protected nucleoside building block in solid-phase oligonucleotide synthesis, enabling the creation of custom DNA or RNA sequences. The 4,4'-dimethoxytrityl (DMT) group protects the 5'-hydroxyl group during synthesis, while the benzoyl group safeguards the exocyclic amine. The 2'-fluoro modification enhances the stability of the resulting oligonucleotides, making them resistant to nuclease degradation. This property is especially valuable in the development of antisense oligonucleotides, siRNA, and other therapeutic nucleic acids for targeted gene regulation and silencing. Its application is crucial in drug discovery, diagnostics, and the study of gene function.