5'-Deoxyadenosine is primarily utilized in biochemical research and pharmaceutical development. It serves as a crucial intermediate in the synthesis of nucleoside analogs, which are widely used in antiviral and anticancer therapies. Its role in modulating DNA and RNA synthesis makes it valuable for studying enzymatic processes and cellular mechanisms. Additionally, it is employed in the development of prodrugs, enhancing the delivery and efficacy of therapeutic agents. In molecular biology, it aids in understanding metabolic pathways and nucleotide metabolism, contributing to advancements in genetic and metabolic disorder treatments.