Used extensively in oligonucleotide synthesis, particularly in the production of modified RNA sequences. It serves as a protected ribonucleoside phosphoramidite, enabling the controlled assembly of RNA strands. Its application is crucial in research areas like gene expression studies, RNA interference, and the development of therapeutic oligonucleotides. The DMT (dimethoxytrityl) group protects the 5'-hydroxyl, while the DMF (dimethylformamidine) group safeguards the exocyclic amine, ensuring selective reactivity during synthesis. This compound is vital in creating precise RNA molecules for diagnostics, drug development, and molecular biology research.