This compound is primarily used in the synthesis of oligonucleotides, particularly in the field of molecular biology and genetic research. It serves as a key building block in solid-phase oligonucleotide synthesis, where it is employed to introduce 2'-O-methyluridine residues into RNA sequences. The 4,4'-dimethoxytrityl (DMT) group protects the 5'-hydroxyl during synthesis, while the 2-cyanoethyl and diisopropyl groups on the phosphoramidite moiety ensure efficient coupling reactions. Its application is crucial in creating modified RNA molecules for therapeutic purposes, such as antisense oligonucleotides, siRNA, and other RNA-based therapeutics, where enhanced stability and specificity are required. Additionally, it is utilized in the development of diagnostic probes and tools for studying gene function and regulation.