This chemical is primarily used in the synthesis of oligonucleotides, particularly in the field of molecular biology and genetic research. It serves as a protected nucleoside building block during the solid-phase synthesis of DNA or RNA sequences. The dimethoxytrityl (DMT) group protects the 5'-hydroxyl group, while the isobutyryl group protects the exocyclic amine of the guanine base, ensuring selective reactivity during the coupling steps. This compound is essential for creating precise and high-quality oligonucleotides, which are widely used in applications such as PCR, gene editing, sequencing, and the development of therapeutic nucleic acids. Its role in enabling controlled and efficient synthesis makes it a critical component in advancing biotechnology and pharmaceutical research.