Used primarily in oligonucleotide synthesis, it serves as a protected nucleoside to ensure selective reactivity during the formation of RNA strands. The DMT (dimethoxytrityl) group protects the 5'-hydroxyl, while the TBDMS (tert-butyldimethylsilyl) group shields the 2'-hydroxyl, allowing precise control over the assembly of RNA sequences. This compound is critical in the production of synthetic RNA for research, therapeutics, and diagnostics, including applications in siRNA, antisense oligonucleotides, and mRNA vaccines. Its protective groups are later removed under specific conditions to yield the desired RNA product.