This chemical is primarily used in the synthesis of oligonucleotides, particularly in the field of molecular biology and genetic research. It serves as a protected nucleoside building block during the solid-phase synthesis of DNA sequences. The dimethoxytrityl (DMT) group acts as a protecting group for the 5'-hydroxyl, ensuring selective reactivity during the coupling steps, while the acetyl group protects the exocyclic amine, preventing unwanted side reactions. The iodine atom at the 5-position allows for further functionalization or cross-coupling reactions, making it valuable in the creation of modified nucleic acids for applications such as antisense therapy, gene editing, and the development of diagnostic probes. Its role is critical in producing high-purity, site-specifically modified oligonucleotides for advanced biomedical research.