3'-Azido-3'-deoxy-beta-L-uridine is primarily utilized in biochemical research as a nucleoside analog. It is often employed in the study of nucleic acid metabolism and the mechanisms of antiviral agents. This compound is particularly valuable in the investigation of RNA synthesis and processing, as it can be incorporated into RNA strands to study the effects of modified nucleosides on RNA function and stability. Additionally, it serves as a tool in the development of antiviral drugs, where its incorporation into viral RNA can inhibit viral replication by disrupting the normal function of viral polymerases. Researchers also use it to explore the biochemical pathways involved in nucleotide salvage and the cellular response to nucleoside analogs, providing insights into potential therapeutic strategies for viral infections and other diseases.