Used in biochemical and molecular biology research as a modified nucleotide analog to study enzyme mechanisms, particularly in RNA synthesis and phosphorylation processes. Serves as a substrate or inhibitor for kinases and other nucleotide-dependent enzymes. Its thiophosphate group enhances resistance to degradation, making it valuable in metabolic stability studies and in the development of therapeutic oligonucleotides. Commonly employed in assays involving G-protein signaling and nucleotide-binding proteins due to its altered electronic and structural properties.