Used in organic synthesis as a nucleoside intermediate, particularly in the preparation of modified RNA or DNA analogs, including antiviral agents targeting viruses like HCV. The trimethylsilyl ethynyl group serves as a protected alkyne for further functionalization via click chemistry or palladium-catalyzed coupling reactions, making it valuable in the development of antisense oligonucleotides, probes for bioconjugation, and nucleic acid therapeutics such as nucleotide analogs that inhibit viral RNA polymerases. This compound is commonly employed in medicinal chemistry for constructing oligonucleotide-based drugs and diagnostic tools with high efficacy and low side effects, enabling selective action in infected cells.