Used in solid-phase oligonucleotide synthesis, particularly for the preparation of RNA strands. The DMT (dimethoxytrityl) group protects the 5'-hydroxyl during chain assembly, allowing stepwise addition of nucleotides and enabling monitoring of coupling efficiency through trityl cation release. The TBDMS (tert-butyldimethylsilyl) group selectively protects the 2'-hydroxyl, providing stability and regioselectivity during synthesis while being removable under mild conditions post-assembly. The benzoyl (Bz) group protects the cytosine base, minimizing side reactions. This modified nucleoside is essential for producing high-purity RNA for research, diagnostics, and therapeutic applications such as antisense oligonucleotides, siRNA, and CRISPR guide RNAs.