Widely used in oligonucleotide synthesis, this compound serves as a key building block for the preparation of RNA sequences. The 5’-O-(4,4’-dimethoxytrityl) group acts as a protecting group for the 5’-hydroxyl, enabling selective deprotection and stepwise chain elongation during solid-phase synthesis. The 2’-O-t-butyldimethylsilyl group protects the 2’-hydroxyl of the ribose sugar, preventing unwanted side reactions and ensuring correct phosphodiester bond formation. Its selective stability and cleavage characteristics make it especially valuable in the synthesis of long or modified RNA strands used in research, diagnostics, and therapeutic applications such as antisense technology and RNA interference.