Used in the synthesis of modified RNA molecules for therapeutic applications, particularly in mRNA vaccine development and antisense oligonucleotide technologies. The 2'-O-methyl modification enhances RNA stability by reducing degradation from nucleases, while the N4-benzoyl group acts as a protecting group during solid-phase synthesis, improving coupling efficiency and yield. This modified nucleoside helps fine-tune the immunogenicity and translational efficiency of synthetic RNA, making it valuable in advanced nucleic acid-based drugs and research tools.