N4-Benzoylcytidine is a protected nucleoside derivative used primarily in the solid-phase synthesis of RNA oligonucleotides. The N4-benzoyl group serves as a protecting moiety for the exocyclic amino group of cytidine, preventing unwanted side reactions during coupling steps and enabling the incorporation of cytidine into synthetic RNA sequences. It is widely employed in biochemical research to study RNA structure, function, and modifications, including the creation of oligonucleotides with improved stability, nuclease resistance, or targeted binding properties. Additionally, it acts as a key intermediate in the development of therapeutic nucleic acids for applications in antiviral therapies, anticancer agents, and treatments for genetic disorders by interfering with nucleic acid replication or gene expression.