Used in oligonucleotide synthesis as a protected guanosine derivative, enabling controlled DNA or RNA strand assembly. The DMT (dimethoxytrityl) group protects the 5'-hydroxyl during solid-phase synthesis, allowing stepwise addition of nucleotides. The acetyl group stabilizes the exocyclic amine of guanine, preventing side reactions. After each coupling step, the DMT group can be selectively removed under mild acidic conditions, facilitating real-time monitoring of synthesis progress through colorimetric detection. Commonly employed in automated synthesizers for producing antisense oligonucleotides, primers, and probes used in molecular biology, diagnostics, and therapeutic development.