Used in research as a nucleoside analog to study RNA synthesis and function. It serves as a building block in the development of modified RNA molecules for therapeutic applications, including antisense oligonucleotides and RNA-based drugs. Also employed in biochemical assays to investigate enzyme activity, particularly in kinases and phosphorylases involved in nucleotide metabolism. Its fluorinated structure enhances stability and binding affinity, making it valuable in probing nucleic acid interactions and designing inhibitors for viral or cancer targets.