Used in molecular biology research as a modified nucleoside analog to study DNA and RNA synthesis. It serves as a building block in the preparation of oligonucleotides with altered base-pairing properties, enhancing stability against nucleases. Commonly applied in antisense technology, PCR optimization, and the development of therapeutic nucleic acids where resistance to degradation is critical. Also utilized in structural studies of nucleic acids due to its ability to influence duplex stability and conformation.