Used in the synthesis of modified oligonucleotides for antisense therapy and diagnostic probes due to its ability to enhance nuclease resistance and binding affinity to complementary RNA. Serves as a building block in the preparation of nucleic acid analogs for studying enzyme mechanisms, particularly in DNA polymerase and repair enzyme research. Also employed in the development of aptamers with improved stability and in bioconjugation strategies where the carboxylic acid group allows for easy coupling to labels, surfaces, or carrier molecules.