7-Deaza-2'-deoxyadenosine is a nucleoside analog used in molecular biology research, particularly for DNA sequencing. Its structure resembles deoxyadenosine but features a carbon atom at the 7-position of the purine ring, conferring resistance to certain enzymatic cleavages. This makes it suitable for controlling DNA synthesis in laboratory settings. It is commonly employed in Sanger sequencing to enhance accuracy in base sequence reading, especially in complex DNA structures like high GC-content regions. Additionally, it reduces compression bands in electrophoresis, resulting in clearer sequencing outcomes. It is also utilized in PCR and other amplification techniques to improve specificity and minimize off-target effects.