Used in molecular biology for in vitro synthesis of capped RNA transcripts, enabling efficient translation in eukaryotic systems. Acts as a cap analog during RNA transcription, ensuring proper 5′ capping which enhances mRNA stability, promotes ribosomal binding, and reduces immune recognition. Commonly applied in vaccine development, gene expression studies, and functional genomics where high translational efficiency and RNA integrity are critical. Preferred in co-transcriptional capping to improve yield and functionality of synthetic mRNA.