Used primarily in biochemical research, 2,2'-O-Cyclouridine serves as a crucial intermediate in the synthesis of modified nucleosides. It plays a significant role in studying RNA structure and function, particularly in the development of RNA-based therapeutics. Its application extends to the investigation of enzymatic processes involving RNA, aiding in the understanding of RNA metabolism and the design of antiviral and anticancer drugs. Additionally, it is utilized in the preparation of stable RNA analogs for structural and mechanistic studies, contributing to advancements in molecular biology and drug discovery.