2'-O-Methylguanosine is primarily used in research and pharmaceutical applications, particularly in the study of RNA structure and function. It plays a significant role in RNA modification studies, helping to understand the impact of methylation on RNA stability, translation, and gene expression. This compound is also utilized in the development of nucleotide analogs for therapeutic purposes, such as antiviral or anticancer drugs, where modified nucleosides can enhance drug efficacy or reduce side effects. Additionally, it serves as a valuable tool in synthetic biology for constructing modified RNA molecules with specific properties for biotechnological applications.