This compound is primarily used in medical research and oncology as an antimetabolite and chemotherapeutic agent. It inhibits DNA synthesis by interfering with the replication process, making it effective in the treatment of certain types of leukemia and other cancers. Additionally, it is utilized in laboratory studies to induce cell cycle arrest, particularly in the S phase, allowing researchers to study cellular mechanisms and responses to DNA damage. Its role in modulating cell proliferation also extends to its use in understanding viral replication and developing antiviral strategies.