3'-O-Methylguanosine is primarily utilized in biochemical research as a marker for studying RNA metabolism and modification processes. It plays a significant role in identifying and analyzing RNA modifications, particularly in understanding the mechanisms of RNA processing and degradation. This compound is also employed in the development of diagnostic tools for detecting RNA-related diseases, as its presence can indicate specific RNA alterations. Additionally, it serves as a reference standard in analytical techniques such as liquid chromatography-mass spectrometry (LC-MS) to ensure accurate quantification of modified nucleosides in biological samples. Its application extends to exploring the impact of RNA modifications on gene expression and cellular function, contributing to advancements in molecular biology and therapeutic research.