Used in biochemical research to study DNA and RNA modifications, particularly in understanding methylation processes. It serves as a reference compound in analytical techniques like HPLC and mass spectrometry to identify methylated nucleobases. Also employed in enzymatic studies to investigate the activity of methyltransferases and other enzymes involved in nucleic acid metabolism. Its role in epigenetic research helps explore gene expression regulation and cellular differentiation. Additionally, it aids in the development of nucleoside analogs for potential therapeutic applications.