pBR322 Plasmid DNA is widely used in molecular biology as a cloning vector. It serves as a tool for gene cloning, allowing researchers to insert, amplify, and manipulate specific DNA sequences in host organisms like E. coli. Its unique antibiotic resistance markers, such as ampicillin and tetracycline, enable the selection of transformed cells. Additionally, it is employed in studies involving recombinant DNA technology, gene expression analysis, and the production of proteins. Its well-characterized structure and multiple cloning sites make it a versatile choice for genetic engineering and biotechnological applications.