Used primarily in biochemical and pharmacological research as a fluorescent probe for detecting and quantifying nucleic acids, particularly DNA and RNA. Its strong affinity for nucleic acids and significant fluorescence enhancement upon binding make it valuable in cell viability assays, apoptosis studies, and flow cytometry. Also employed in studies of membrane permeability due to its ability to cross cell membranes and stain intracellular nucleic acids. Shows potential as a scaffold in developing anticancer agents due to its intercalating properties with DNA, which can interfere with DNA replication and transcription.