Ampliflu Red is a fluorescent dye primarily used in molecular biology for real-time PCR (qPCR) applications to detect and quantify nucleic acids, particularly DNA. It binds to double-stranded DNA generated during amplification, emitting bright red fluorescence for real-time monitoring of gene amplification. This provides high sensitivity and clear signals even at low concentrations, enabling accurate quantification.

It serves as a safer alternative to traditional dyes like ethidium bromide, with reduced mutagenicity and enhanced stability. Ampliflu Red is compatible with various commercial qPCR systems and downstream applications such as cloning or sequencing, making it suitable for research laboratories, medical labs, infectious disease diagnostics (e.g., virus or bacteria detection), genetic analysis, and quality control in nucleic acid studies.