Used extensively in molecular biology for staining DNA in fluorescence microscopy and flow cytometry. It binds selectively to the minor groove of double-stranded DNA, emitting blue fluorescence under UV light, making it ideal for visualizing chromosomal structures and nuclear morphology. Commonly employed in cell viability assays to distinguish between live and dead cells based on DNA integrity. Also utilized in research for detecting apoptotic cells and studying DNA-protein interactions due to its high affinity for AT-rich regions. Its stability and specificity make it a valuable tool in genetic and cytogenetic studies.