This DNA stain is widely used in molecular biology for visualizing DNA in agarose gels during electrophoresis. It binds specifically to DNA and fluoresces under blue light, allowing for easy detection and analysis of DNA fragments. The stain is highly sensitive, making it ideal for identifying even small quantities of DNA. It is commonly applied in genetic research, PCR product verification, and cloning experiments. Its compatibility with blue light excitation ensures safer handling compared to UV light-based stains. Additionally, it is often used in educational settings for teaching DNA analysis techniques due to its ease of use and clear visualization.