Used extensively in molecular biology for staining nucleic acids in agarose gels, it provides high sensitivity and low background fluorescence, making it ideal for visualizing DNA and RNA fragments during electrophoresis. Its application extends to quantitative PCR (qPCR) for detecting amplified DNA in real-time due to its ability to bind to double-stranded DNA and emit fluorescence. Researchers also utilize it in fluorescence microscopy to study nucleic acid localization within cells. However, caution is advised as it is a potent mutagen and requires proper handling and disposal. Its compatibility with standard UV transilluminators and blue-light transilluminators enhances its versatility in various laboratory settings.