Used in molecular biology to efficiently release purified plasmid DNA from silica-based spin columns during nucleic acid purification. This buffer disrupts the interaction between the DNA and the membrane, allowing the DNA to elute in a small volume for downstream applications such as PCR, restriction digestion, transfection, and sequencing. Its optimized pH and ionic conditions ensure high recovery and integrity of supercoiled plasmid DNA, making it essential for cloning and gene expression workflows.